Rhodopsin kinase: Expression in mammalian cells and a two-step purification

A suitable system for expression of the rhodopsin kinase (RK) gene and its mutants is needed for structure–function studies of RK. Previously, investigation of the baculovirus system showed satisfactory production of RK, but posttranslational isoprenylation was deficient. We now report on a comparat...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Bruel, Christophe, Cha, Kiweon, Reeves, Philip J., Getmanova, Elena, Khorana, H. Gobind
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2000
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC16182/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10737781/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.7.3004
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