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Catalytic Core of Alphavirus Nonstructural Protein nsP4 Possesses Terminal Adenylyltransferase Activity

The RNA-dependent RNA polymerase nsP4 is an integral part of the alphavirus replication complex. To define the role of nsP4 in viral RNA replication and for a structure-function analysis, we expressed Sindbis virus nsP4 in Escherichia coli. The core catalytic domain of nsP4 (Δ97nsP4, a deletion of t...

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Detalhes bibliográficos
Main Authors: Tomar, Shailly, Hardy, Richard W., Smith, Janet L., Kuhn, Richard J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1617302/
https://ncbi.nlm.nih.gov/pubmed/17005674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.01067-06
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