Molecular cloning of the cowpea leghemoglobin II gene and expression of its cDNA in Escherichia coli. Purification and characterization of the recombinant protein.

Cowpea (Vigna unguiculata) nodules contain three leghemoglobins (LbI, LbII, and LbIII) that are encoded by at least two genes. We have cloned and sequenced the gene that encodes for LbII (lbII), the most abundant Lb in cowpea nodules, using total DNA as the template for PCR. Primers were designed us...

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發表在:	Plant Physiol
Principais autores:	Arredondo-Peter, R, Moran, J F, Sarath, G, Luan, P, Klucas, R V
格式:	Artigo
語言:	Inglês
出版:	Oxford University Press 1997
主題:	Research Article
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC158329/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9193085/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.114.2.493
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