Molecular cloning of the cowpea leghemoglobin II gene and expression of its cDNA in Escherichia coli. Purification and characterization of the recombinant protein.

Cowpea (Vigna unguiculata) nodules contain three leghemoglobins (LbI, LbII, and LbIII) that are encoded by at least two genes. We have cloned and sequenced the gene that encodes for LbII (lbII), the most abundant Lb in cowpea nodules, using total DNA as the template for PCR. Primers were designed us...

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Detalhes bibliográficos

Publicado no:	Plant Physiol
Principais autores:	Arredondo-Peter, R, Moran, J F, Sarath, G, Luan, P, Klucas, R V
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC158329/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9193085/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.114.2.493
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