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Extraordinary rates of transition metal ion-mediated ribozyme catalysis
In pre-steady-state, fast-quench kinetic analysis, the tertiary-stabilized hammerhead ribozyme “RzB” cleaves its substrate RNA with maximal measured k (obs) values of ∼3000 min(−1) in 1 mM Mn(2+) and ∼780 min(−1) in 1 mM Mg(2+) at 37°C (pH 7.4). Apparent pKa for the catalytic general base is ∼7.8–8....
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Cold Spring Harbor Laboratory Press
2006
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1581984/ https://ncbi.nlm.nih.gov/pubmed/16912216 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.128906 |
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