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k-Space Image Correlation Spectroscopy: A Method for Accurate Transport Measurements Independent of Fluorophore Photophysics

We present the theory and application of reciprocal space image correlation spectroscopy (kICS). This technique measures the number density, diffusion coefficient, and velocity of fluorescently labeled macromolecules in a cell membrane imaged on a confocal, two-photon, or total internal reflection f...

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Autori principali: Kolin, David L., Ronis, David, Wiseman, Paul W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Biophysical Society 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1578478/
https://ncbi.nlm.nih.gov/pubmed/16861272
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.082768
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