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k-Space Image Correlation Spectroscopy: A Method for Accurate Transport Measurements Independent of Fluorophore Photophysics

We present the theory and application of reciprocal space image correlation spectroscopy (kICS). This technique measures the number density, diffusion coefficient, and velocity of fluorescently labeled macromolecules in a cell membrane imaged on a confocal, two-photon, or total internal reflection f...

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Autors principals: Kolin, David L., Ronis, David, Wiseman, Paul W.
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1578478/
https://ncbi.nlm.nih.gov/pubmed/16861272
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.082768
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