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Quantitative Determination of Free-DNA Uptake in River Bacteria at the Single-Cell Level by In Situ Rolling-Circle Amplification

Detection of plasmid DNA uptake in river bacteria at the single-cell level was carried out by rolling-circle amplification (RCA). Uptake of a plasmid containing the green fluorescent protein gene (gfp) by indigenous bacteria from two rivers in Osaka, Japan, was monitored for 506 h using this in situ...

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Bibliographische Detailangaben
Hauptverfasser: Maruyama, Fumito, Tani, Katsuji, Kenzaka, Takehiko, Yamaguchi, Nobuyasu, Nasu, Masao
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2006
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1563595/
https://ncbi.nlm.nih.gov/pubmed/16957252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03035-05
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