Visualization and Enumeration of Bacteria Carrying a Specific Gene Sequence by In Situ Rolling Circle Amplification

Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Maruyama, Fumito, Kenzaka, Takehiko, Yamaguchi, Nobuyasu, Tani, Katsuji, Nasu, Masao
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2005
Pynciau:
Methods
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317385/
https://ncbi.nlm.nih.gov/pubmed/16332770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.7933-7940.2005
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