Visualization and Enumeration of Bacteria Carrying a Specific Gene Sequence by In Situ Rolling Circle Amplification

Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features...

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Detalhes bibliográficos
Principais autores: Maruyama, Fumito, Kenzaka, Takehiko, Yamaguchi, Nobuyasu, Tani, Katsuji, Nasu, Masao
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317385/
https://ncbi.nlm.nih.gov/pubmed/16332770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.7933-7940.2005
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