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Hydrogen-exchange stability analysis of Bergerac-Src homology 3 variants allows the characterization of a folding intermediate in equilibrium

Amide hydrogen/deuterium exchange rates have been determined for two mutants of α-spectrin Src homology 3 domain (WT), containing an elongated stable (SHH) and unstable (SHA) distal loop. SHA, similarly to WT, follows a two-state transition, whereas SHH apparently folds via a three-state mechanism....

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Detalhes bibliográficos
Main Authors: Viguera, Ana-Rosa, Serrano, Luis
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC156269/
https://ncbi.nlm.nih.gov/pubmed/12719536
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0837456100
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