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DNA relaxation by human topoisomerase I occurs in the closed clamp conformation of the protein

In cocrystal structures of human topoisomerase I and DNA, the enzyme is tightly clamped around the DNA helix. After cleavage and covalent attachment of the enzyme to the 3′ end at the nick, DNA relaxation requires rotation of the DNA helix downstream of the cleavage site. Models based on the cocryst...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Carey, James F., Schultz, Sharon J., Sisson, Lisa, Fazzio, Thomas G., Champoux, James J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The National Academy of Sciences 2003
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC156254/
https://ncbi.nlm.nih.gov/pubmed/12711735
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1031537100
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