Linker-Mediated Recombinational Subcloning of Large DNA Fragments Using Yeast

The homologous recombination pathway in yeast is an ideal tool for the sequence-specific assembly of plasmids. Complementary 80-nucleotide oligonucleotides that overlap a vector and a target fragment were found to serve as efficient recombination linkers for fragment subcloning. Using electroporatio...

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Detaylı Bibliyografya
Asıl Yazarlar: Raymond, Christopher K., Sims, Elizabeth H., Olson, Maynard V.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Cold Spring Harbor Laboratory Press 2002
Konular:
Methods
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC155262/
https://ncbi.nlm.nih.gov/pubmed/11779844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.205201
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