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Linker-Mediated Recombinational Subcloning of Large DNA Fragments Using Yeast
The homologous recombination pathway in yeast is an ideal tool for the sequence-specific assembly of plasmids. Complementary 80-nucleotide oligonucleotides that overlap a vector and a target fragment were found to serve as efficient recombination linkers for fragment subcloning. Using electroporatio...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC155262/ https://ncbi.nlm.nih.gov/pubmed/11779844 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.205201 |
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