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Quantification of Bacteria Adherent to Gastrointestinal Mucosa by Real-Time PCR

The use of real-time quantitative PCR (5′ nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulga...

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書目詳細資料
發表在:J Clin Microbiol
Principais autores: Huijsdens, Xander W., Linskens, Ronald K., Mak, Mariëtte, Meuwissen, Stephan G. M., Vandenbroucke-Grauls, Christina M. J. E., Savelkoul, Paul H. M.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 2002
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在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154607/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12454130/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.12.4423-4427.2002
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