Quantification of Bacteria Adherent to Gastrointestinal Mucosa by Real-Time PCR

The use of real-time quantitative PCR (5′ nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulga...

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Bibliographic Details
Main Authors: Huijsdens, Xander W., Linskens, Ronald K., Mak, Mariëtte, Meuwissen, Stephan G. M., Vandenbroucke-Grauls, Christina M. J. E., Savelkoul, Paul H. M.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2002
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC154607/
https://ncbi.nlm.nih.gov/pubmed/12454130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.12.4423-4427.2002
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