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Quantification of Bacteria Adherent to Gastrointestinal Mucosa by Real-Time PCR
The use of real-time quantitative PCR (5′ nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulga...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC154607/ https://ncbi.nlm.nih.gov/pubmed/12454130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.12.4423-4427.2002 |
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