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The yggH Gene of Escherichia coli Encodes a tRNA (m(7)G46) Methyltransferase
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-l-methionine-dependent formation of N(7)-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant s...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2003
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC154064/ https://ncbi.nlm.nih.gov/pubmed/12730187 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.10.3238-3243.2003 |
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