The yggH Gene of Escherichia coli Encodes a tRNA (m(7)G46) Methyltransferase

We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-l-methionine-dependent formation of N(7)-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant s...

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Hauptverfasser: De Bie, Lara G. S., Roovers, Martine, Oudjama, Yamina, Wattiez, Ruddy, Tricot, Catherine, Stalon, Victor, Droogmans, Louis, Bujnicki, Janusz M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2003
Schlagworte:
Genetics and Molecular Biology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC154064/
https://ncbi.nlm.nih.gov/pubmed/12730187
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.10.3238-3243.2003
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