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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy

The affinity of the origin-binding domain (OBD) of simian virus 40 large T antigen for its cognate origin was measured at equilibrium using a DNA binding assay based on fluorescence anisotropy. At a near-physiological concentration of salt, the affinities of the OBD for site II and the core origin w...

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Udgivet i:J Virol
Principais autores: Titolo, S., Welchner, E., White, P. W., Archambault, J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2003
Fag:
Replication
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC153955/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12692254/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.77.9.5512-5518.2003
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