Detection of anti-topoisomerase I antibodies using a full length human topoisomerase I recombinant protein purified from a baculovirus expression system.

Topoisomerase I (topo I) is a major systemic sclerosis (SSc)-associated autoantigen. A cDNA construct encoding full length human topo I in a recombinant baculovirus transfer vector was used to infect insect cells in culture from which recombinant protein was purified. An ELISA using recombinant prot...

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Main Authors: Whyte, J, Earnshaw, W C, Champoux, J J, Parker, L H, Stewart, L, Hall, N D, McHugh, N J
Format: Artigo
Jezik:Inglês
Izdano: 1995
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1534349/
https://ncbi.nlm.nih.gov/pubmed/7743657
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