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High-throughput approach for detection of DNA bending and flexibility based on cyclization
We have developed a high-throughput approach to the labor-intensive problems of DNA cyclization, which we use to characterize DNA curvature and mechanical properties. The method includes a combinatorial approach to make the DNA constructs needed and automated real-time measurement of the kinetics us...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
The National Academy of Sciences
2003
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC152263/ https://ncbi.nlm.nih.gov/pubmed/12629220 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0530189100 |
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