High-throughput approach for detection of DNA bending and flexibility based on cyclization
We have developed a high-throughput approach to the labor-intensive problems of DNA cyclization, which we use to characterize DNA curvature and mechanical properties. The method includes a combinatorial approach to make the DNA constructs needed and automated real-time measurement of the kinetics us...
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| Опубликовано в:: | Proc Natl Acad Sci U S A |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
2003
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC152263/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12629220/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0530189100 |
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