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High-throughput approach for detection of DNA bending and flexibility based on cyclization

We have developed a high-throughput approach to the labor-intensive problems of DNA cyclization, which we use to characterize DNA curvature and mechanical properties. The method includes a combinatorial approach to make the DNA constructs needed and automated real-time measurement of the kinetics us...

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Autori principali: Zhang, Yongli, Crothers, Donald M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 2003
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC152263/
https://ncbi.nlm.nih.gov/pubmed/12629220
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0530189100
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