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Macromolecular-scale resolution in biological fluorescence microscopy

We demonstrate far-field fluorescence microscopy with a focal-plane resolution of 15–20 nm in biological samples. The 10- to 12-fold multilateral increase in resolution below the diffraction barrier has been enabled by the elimination of molecular triplet state excitation as a major source of photob...

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Detalhes bibliográficos
Main Authors: Donnert, Gerald, Keller, Jan, Medda, Rebecca, Andrei, M. Alexandra, Rizzoli, Silvio O., Lührmann, Reinhard, Jahn, Reinhard, Eggeling, Christian, Hell, Stefan W.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1518808/
https://ncbi.nlm.nih.gov/pubmed/16864773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0604965103
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