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Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins

Fluorescence microscopy is indispensable in many areas of science, but until recently, diffraction has limited the resolution of its lens-based variant. The diffraction barrier has been broken by a saturated depletion of the marker's fluorescent state by stimulated emission, but this approach r...

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Bibliographic Details
Main Authors: Hofmann, Michael, Eggeling, Christian, Jakobs, Stefan, Hell, Stefan W.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2005
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1308899/
https://ncbi.nlm.nih.gov/pubmed/16314572
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0506010102
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