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Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission

The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken. This is accomplished by quenching excited organic molecules at the rim of the focal spot through stimulated emission. Along the optic axis, the...

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Detalhes bibliográficos
Main Authors: Klar, Thomas A., Jakobs, Stefan, Dyba, Marcus, Egner, Alexander, Hell, Stefan W.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC26924/
https://ncbi.nlm.nih.gov/pubmed/10899992
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