A Methyltransferase Targeting Assay Reveals Silencer-Telomere Interactions in Budding Yeast

We have designed a modified version of the Dam identification technique and used it to probe higher-order chromatin structure in Saccharomyces cerevisiae. We fused the bacterial DNA methyltransferase Dam to the DNA-binding domain of TetR and targeted the resulting chimera to Tet operators inserted i...

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Detalhes bibliográficos

Publicado no:	Mol Cell Biol
Principais autores:	Lebrun, Eleonore, Fourel, Geneviève, Defossez, Pierre-Antoine, Gilson, Eric
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis 2003
Assuntos:	DNA Dynamics and Chromosome Structure
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC151690/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12588971/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/MCB.23.5.1498-1508.2003
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