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A Methyltransferase Targeting Assay Reveals Silencer-Telomere Interactions in Budding Yeast
We have designed a modified version of the Dam identification technique and used it to probe higher-order chromatin structure in Saccharomyces cerevisiae. We fused the bacterial DNA methyltransferase Dam to the DNA-binding domain of TetR and targeted the resulting chimera to Tet operators inserted i...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2003
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC151690/ https://ncbi.nlm.nih.gov/pubmed/12588971 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.23.5.1498-1508.2003 |
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