Targeted mutagenesis of DNA with alkylating RecA assisted oligonucleotides.
Site-specific mutation was demonstrated in a shuttle vector system using nitrogen mustard-conjugated oligodeoxyribonucleotides (ODNs). Plasmid DNA was modified in vitro by ODNs containing all four DNA bases in the presence of Escherichia coli RecA protein. Up to 50% of plasmid molecules were alkylat...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , , , , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1999
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148761/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10572190/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.24.e38 |
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