Targeted mutagenesis of DNA with alkylating RecA assisted oligonucleotides.

Site-specific mutation was demonstrated in a shuttle vector system using nitrogen mustard-conjugated oligodeoxyribonucleotides (ODNs). Plasmid DNA was modified in vitro by ODNs containing all four DNA bases in the presence of Escherichia coli RecA protein. Up to 50% of plasmid molecules were alkylat...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Singer, M J, Podyminogin, M A, Metcalf, M A, Reed, M W, Brown, D A, Gamper, H B, Meyer, R B, Wydro, R M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1999
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148761/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10572190/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.24.e38
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