Targeted mutagenesis of DNA with alkylating RecA assisted oligonucleotides.
Site-specific mutation was demonstrated in a shuttle vector system using nitrogen mustard-conjugated oligodeoxyribonucleotides (ODNs). Plasmid DNA was modified in vitro by ODNs containing all four DNA bases in the presence of Escherichia coli RecA protein. Up to 50% of plasmid molecules were alkylat...
Kaydedildi:
| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , , , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1999
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148761/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10572190/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.24.e38 |
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