Targeted mutagenesis of DNA with alkylating RecA assisted oligonucleotides.

Site-specific mutation was demonstrated in a shuttle vector system using nitrogen mustard-conjugated oligodeoxyribonucleotides (ODNs). Plasmid DNA was modified in vitro by ODNs containing all four DNA bases in the presence of Escherichia coli RecA protein. Up to 50% of plasmid molecules were alkylat...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Singer, M J, Podyminogin, M A, Metcalf, M A, Reed, M W, Brown, D A, Gamper, H B, Meyer, R B, Wydro, R M
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1999
Aiheet:
Research Article
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC148761/
https://ncbi.nlm.nih.gov/pubmed/10572190
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