Mutational analysis of Escherichia coli DNA ligase identifies amino acids required for nick-ligation in vitro and for in vivo complementation of the growth of yeast cells deleted for CDC9 and LIG4.
We report that the NAD-dependent Escherichia coli DNA ligase can support the growth of Saccharomyces cerevisiae strains deleted singly for CDC9 or doubly for CDC9 plus LIG4. Alanine-scanning mutagenesis of E.coli DNA ligase led to the identification of seven amino acids (Lys115, Asp117, Asp285, Lys3...
Salvato in:
| Pubblicato in: | Nucleic Acids Res |
|---|---|
| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1999
|
| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148661/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10497258/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.20.3953 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|