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Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum

We report the production, purification and characterization of a DNA ligase encoded by the thermophilic archaeon Methanobacterium thermoautotrophicum. The 561 amino acid Mth ligase catalyzed strand-joining on a singly nicked DNA in the presence of a divalent cation (magnesium, manganese or cobalt) a...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Sriskanda, Verl, Kelman, Zvi, Hurwitz, Jerard, Shuman, Stewart
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2000
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC102631/
https://ncbi.nlm.nih.gov/pubmed/10871342
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