Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum

We report the production, purification and characterization of a DNA ligase encoded by the thermophilic archaeon Methanobacterium thermoautotrophicum. The 561 amino acid Mth ligase catalyzed strand-joining on a singly nicked DNA in the presence of a divalent cation (magnesium, manganese or cobalt) a...

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Publicado en:	Nucleic Acids Res
Autores principales:	Sriskanda, Verl, Kelman, Zvi, Hurwitz, Jerard, Shuman, Stewart
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Oxford University Press 2000
Materias:	Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102631/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871342/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.11.2221
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