Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum

We report the production, purification and characterization of a DNA ligase encoded by the thermophilic archaeon Methanobacterium thermoautotrophicum. The 561 amino acid Mth ligase catalyzed strand-joining on a singly nicked DNA in the presence of a divalent cation (magnesium, manganese or cobalt) a...

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Bibliographic Details

Published in:	Nucleic Acids Res
Main Authors:	Sriskanda, Verl, Kelman, Zvi, Hurwitz, Jerard, Shuman, Stewart
Format:	Artigo
Language:	Inglês
Published:	Oxford University Press 2000
Subjects:	Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102631/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871342/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.11.2221
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