Mutational analysis of Escherichia coli DNA ligase identifies amino acids required for nick-ligation in vitro and for in vivo complementation of the growth of yeast cells deleted for CDC9 and LIG4.

We report that the NAD-dependent Escherichia coli DNA ligase can support the growth of Saccharomyces cerevisiae strains deleted singly for CDC9 or doubly for CDC9 plus LIG4. Alanine-scanning mutagenesis of E.coli DNA ligase led to the identification of seven amino acids (Lys115, Asp117, Asp285, Lys3...

Description complète

Enregistré dans:
Détails bibliographiques
Auteurs principaux: Sriskanda, V, Schwer, B, Ho, C K, Shuman, S
Format: Artigo
Langue:Inglês
Publié: 1999
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC148661/
https://ncbi.nlm.nih.gov/pubmed/10497258
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!

Documents similaires