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Polymorphism analysis and gene detection by minisequencing on an array of gel-immobilized primers.

Two procedures, multibase and multiprimer, have been developed for single nucleotide extension of primers immobilized within polyacrylamide gel pads on a microchip. In the multibase assay, a primer is next to a polymorphic nucleotide; the nucleotide is identified by the specificity with which the pr...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Dubiley, S, Kirillov, E, Mirzabekov, A
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1999
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148611/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10471749/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.18.e19
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