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Polymorphism analysis and gene detection by minisequencing on an array of gel-immobilized primers.

Two procedures, multibase and multiprimer, have been developed for single nucleotide extension of primers immobilized within polyacrylamide gel pads on a microchip. In the multibase assay, a primer is next to a polymorphic nucleotide; the nucleotide is identified by the specificity with which the pr...

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Detalles Bibliográficos
Main Authors: Dubiley, S, Kirillov, E, Mirzabekov, A
Formato: Artigo
Idioma:Inglês
Publicado: 1999
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC148611/
https://ncbi.nlm.nih.gov/pubmed/10471749
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