Simplified generation of targeting constructs using ET recombination.

ET recombination is a way to engineer DNA in Escherichia coli using homologous recombination. Here we develop the potential of ET recombination in two ways relevant to complex engineering exercises such as building gene targeting constructs. First, a targeting construct was made in a single step. Se...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Angrand, P O, Daigle, N, van der Hoeven, F, Schöler, H R, Stewart, A F
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1999
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148577/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10446259/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.17.e16
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