Simplified generation of targeting constructs using ET recombination.

ET recombination is a way to engineer DNA in Escherichia coli using homologous recombination. Here we develop the potential of ET recombination in two ways relevant to complex engineering exercises such as building gene targeting constructs. First, a targeting construct was made in a single step. Se...

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Библиографические подробности
Главные авторы: Angrand, P O, Daigle, N, van der Hoeven, F, Schöler, H R, Stewart, A F
Формат: Artigo
Язык:Inglês
Опубликовано: 1999
Предметы:
Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC148577/
https://ncbi.nlm.nih.gov/pubmed/10446259
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