Simplified generation of targeting constructs using ET recombination.
ET recombination is a way to engineer DNA in Escherichia coli using homologous recombination. Here we develop the potential of ET recombination in two ways relevant to complex engineering exercises such as building gene targeting constructs. First, a targeting construct was made in a single step. Se...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1999
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148577/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10446259/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.17.e16 |
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