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Enzymatic mutation detection: enrichment of heteroduplexes from hybrid DNA mixtures by cleavage-deficient GST-tagged endonuclease VII.

A method for the enrichment of heteroduplex DNAs from hybrid DNA mixtures by endonuclease VII is reported. The procedure is based on the ability of a GST-fused cleavage-deficient mutant endonuclease VII (EVII-N62D(GST)) to bind to mismatching nucleotides in heteroduplex DNAs identical to the wild-ty...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Golz, S, Kemper, B
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1999
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC148518/
https://ncbi.nlm.nih.gov/pubmed/10454626
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