Nucleotide analogs and new buffers improve a generalized method to enrich for low abundance mutations.
A high sensitivity method for detecting low level mutations is under development. A PCR reaction is performed in which a restriction site is introduced in wild-type DNA by alteration of specific bases. Digestion of wild-type DNA by the cognate restriction endonuclease (RE) enriches for products with...
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| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1999
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148389/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10101189/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.8.1819 |
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