Nucleotide analogs and new buffers improve a generalized method to enrich for low abundance mutations.

A high sensitivity method for detecting low level mutations is under development. A PCR reaction is performed in which a restriction site is introduced in wild-type DNA by alteration of specific bases. Digestion of wild-type DNA by the cognate restriction endonuclease (RE) enriches for products with...

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Bibliografische gegevens

Gepubliceerd in:	Nucleic Acids Res
Hoofdauteurs:	Day, J P, Hammer, R P, Bergstrom, D, Barany, F
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Oxford University Press 1999
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148389/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10101189/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.8.1819
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