An improved method for routine preparation of intact artificial chromosome DNA (340-1000 kb) for transfection into human cells.
The transfer of high molecular weight (HMW) DNA into mammalian cells is an important strategy for assessing human gene expression and chromosome structure and function. However, using current methods, it is difficult to dependably prepare intact HMW DNA because of the susceptibility of the DNA to de...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1999
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148381/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10076009/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.7.1762 |
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