An improved method for routine preparation of intact artificial chromosome DNA (340-1000 kb) for transfection into human cells.

The transfer of high molecular weight (HMW) DNA into mammalian cells is an important strategy for assessing human gene expression and chromosome structure and function. However, using current methods, it is difficult to dependably prepare intact HMW DNA because of the susceptibility of the DNA to de...

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Podrobná bibliografie

Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Compton, S T, Henning, K A, Chen, M, Mansoura, M K, Ashlock, M A
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 1999
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148381/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10076009/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.7.1762
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