An improved method for routine preparation of intact artificial chromosome DNA (340-1000 kb) for transfection into human cells.

The transfer of high molecular weight (HMW) DNA into mammalian cells is an important strategy for assessing human gene expression and chromosome structure and function. However, using current methods, it is difficult to dependably prepare intact HMW DNA because of the susceptibility of the DNA to de...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Compton, S T, Henning, K A, Chen, M, Mansoura, M K, Ashlock, M A
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1999
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148381/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10076009/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.7.1762
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