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New device and method for capture, reverse transcription and nested PCR in a single closed-tube.

A device and improved method based on the use of a compartmentalized Eppendorf tube that allows capture, reverse transcription and nested-PCR in a single closed-tube has been developed and patented. The main advantages of the system are the high sensitivity obtained, the simplicity, the low risk of...

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Detalhes bibliográficos
Main Authors: Olmos, A, Cambra, M, Esteban, O, Gorris, M T, Terrada, E
Formato: Artigo
Idioma:Inglês
Publicado em: 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC148356/
https://ncbi.nlm.nih.gov/pubmed/10037824
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