Reverse transcription polymerase chain reaction (RT‐PCR) for diagnosis of respiratory syncytial virus infection in adults: Use of a single‐tube “hanging droplet” nested PCR

Rapid diagnosis of Respiratory Syncytial virus (RSV) infection is difficult in elderly persons due to the low quantities of virus shed. Therefore, reverse transcription‐polymerase chain reaction (RT‐PCR) was used to detect viral RNA in respiratory secretions. A single‐tube nested RT‐PCR that used pr...

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Bibliographic Details
Published in:J Med Virol
Main Authors: Walsh, Edward E., Falsey, Ann R., Swinburne, Ian A., Formica, Maria A.
Format: Artigo
Language:Inglês
Published: John Wiley & Sons, Inc. 2001
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7166324/
https://ncbi.nlm.nih.gov/pubmed/11170067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1096-9071(200103)63:3<259::AID-JMV1010>3.0.CO;2-X
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