Amplification of cDNA ends based on template-switching effect and step-out PCR.

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to ampl...

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Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Matz, M, Shagin, D, Bogdanova, E, Britanova, O, Lukyanov, S, Diatchenko, L, Chenchik, A
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 1999
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148354/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10037822/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.6.1558
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