Amplification of cDNA ends based on template-switching effect and step-out PCR.
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to ampl...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , , , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1999
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148354/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10037822/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.6.1558 |
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