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Amplification of cDNA ends based on template-switching effect and step-out PCR.

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to ampl...

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Publicado no:Nucleic Acids Res
Principais autores: Matz, M, Shagin, D, Bogdanova, E, Britanova, O, Lukyanov, S, Diatchenko, L, Chenchik, A
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1999
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148354/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10037822/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.6.1558
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