Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display.

We have developed a method for high-efficiency window separation of cDNA display by increasing the specificity of priming in reverse transcription. In the conventional method, two-base anchored oligo(dT) primers (5'dT16VN3', where N is any base and V is G, A or C) are used to make windows for the di...

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Detaylı Bibliyografya
Yayımlandı:	Nucleic Acids Res
Asıl Yazarlar:	Mizuno, Y, Carninci, P, Okazaki, Y, Tateno, M, Kawai, J, Amanuma, H, Muramatsu, M, Hayashizaki, Y
Materyal Türü:	Artigo
Dil:	Inglês
Baskı/Yayın Bilgisi:	Oxford University Press 1999
Konular:	Research Article
Online Erişim:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148322/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9973624/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.5.1345
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Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display.

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